Últimas publicaciones científicas ISI
Chryseobacterium oncorhynchi sp. nov., isolated from rainbow trout
(Oncorhynchus mykiss)
Systematic And Applied Microbiology 35(1):24-9.
Genotypic and phenotypic analyses were performed on five Gram-negative, catalase and oxidasepositive, rod-shaped bacteria isolated from the gill and liver of four rainbow trout. Studies based on comparative 16S rRNA gene sequence analysis showed that the five new isolates shared 99.8–100% sequence similarity and that they belong to the genus Chryseobacterium. The nearest phylogenetic neighbours of the strain 701B-08T were Chryseobacterium ureilyticum F-Fue-04IIIaaaaT (99.1% 16S rRNA gene sequence similarity) and Chryseobacterium joosteii LMG 18212T (98.6%). DNA–DNA hybridization values between the five isolates were 91–99% and ranged from 2 to 53% between strain 701B-08T and the type strains of phylogenetically closely related species of Ch… Leer más
Zamora L., Fernandez-Garayzabal JF., Palacios MA., Sanchez-Porro C., Svensson-Stadler LA., Dominguez L., Moore ERB., Ventosa A. y Vela AI..
Tuberculosis due to Mycobacterium bovis and Mycobacterium caprae in sheep
The Veterinary Journal 191(2):267-9.
Tuberculosis was diagnosed in three flocks of sheep in Galicia, Spain, in 2009 and 2010. Two flocks were infected with Mycobacterium bovis and one flock was infected with Mycobacterium caprae. Infection was confirmed by the comparative intradermal tuberculin test, bacteriology, molecular analysis and histopathology. Sheep have the potential to act as a reservoir for tuberculosis… Leer más
Munoz-Mendoza M., de Juan L., Menendez S., Ocampo A., Mourelo J., Saez JL., Dominguez L., Gortazar C., Garcia-Marin JF. y Balseiro A.
First isolation of the anamorph of Kazachstania heterogenica from a fatal infection in a primate host
Medical Mycology
50(2):193-6.
We describe the isolation of the anamorph of the ascomycetous yeast Kazachstania heterogenica from a fatal infection in a 2 year, 9-month-old female white-handed gibbon (Hylobates lar). The yeast was observed in histological sections (lung and intestine) and co-isolated with the bacterium Escherichia coli from different internal organs. This is the first report of the recovery of this yeast from a fatal infection in a primate host… Leer más
Alvarez-Perez S., Mateos A., Dominguez L., Martinez-Nevado E., Rodriguez-Bertos A., Blanco JL. y Garcia ME.
Diagnosis of Tuberculosis in Camelids: Old Problems, Current Solutions and Future Challenges
Transboundary And Emerging Diseases 59(1):1-10.
In spite of great efforts for its control and eradication, tuberculosis remains one of the most important zoonosis worldwide. Its causative agents, the members of the Mycobacterium tuberculosis complex, have a wide host range that complicates the epidemiology of this disease. Among susceptible species to these pathogens, camelids from the New World (llama, alpaca and vicuña) and Old World (Bactrian camel and dromedary) are acquiring an increasing importance in several European countries because of its growing number and could act as reservoirs of the disease for livestock and humans in their natural habitat. In addition, tuberculosis caused by a number of M. tuberculosis complex members is a life-threatening disease in these animal s… Leer más
Alvarez J., Bezos J., de Juan L., Vordermeier M., Rodriguez-Campos S., Fernandez de Mera IG., Mateos A. y Dominguez L..
Tuberculosis in goats: Assessment of current in vivo cell-mediated and antibody-based diagnostic assays
The Veterinary Journal 191(2):161-5.
Tuberculosis in goats, mainly caused by Mycobacterium bovis and M. caprae, is a zoonotic disease with implications for public health, as well as having an economic impact due to decreased goat production, increased mortality rates and costs of diagnosis. There is an increasing need for surveillance of tuberculosis-infected goat herds, particularly in countries that are not officially free of bovine tuberculosis, and goats sharing farms with cattle should be subjected to the official tuberculin test. In Spain, some regions have programmes for the control of tuberculosis in goats, applying the same diagnostic assays that are used for cattle. The objective of tuberculosis eradication in livestock requires adaptation of existing control strateg… Leer más
Bezos J., Alvarez J., Romero B., Aranaz A. y de Juan L..
Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats
Veterinary Microbiology 154(3-4):292-7.
A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected 57/60 SICTT (95.0%) positive animals in one M. bovis infected herd and 120/120 (100%) in a second herd. In a separate experiment, 28 M. caprae culture… Leer más
Shuralev E., Quinn P., Doyle M., Duignan A., Kwok HF., Bezos J., Olwill SA., Gormley E., Aranaz A., Good M., Davis WC., Clarke J. y Whelan C.
Septicemic salmonellosis caused by Salmonella Hessarek in wintering and migrating song thrushes (Turdus philomelos) in Spain
Journal Of Wildlife Diseases 48(1):113-121.
We investigated two mortality events in wintering and migrating Song Thrushes (Turdus philomelos) in Catalonia, northeastern Spain in 2009 and 2010. Both episodes occurred in late February to mid-March during the spring migration. Salmonellosis produced by the serotype Salmonella enterica subsp. enterica serotype Hessarek (S. Hessarek) was identified as the cause of death in both episodes. Poor body condition, marked splenomegaly, and microscopic disseminated intravascular coagulation with numerous intravascular and tissular bacteria were the most consistent findings. Macro-restriction profiling by pulsed-field gel electrophoresis (PFGE) using XbaI was performed for epidemiologic typing of the S. Hessarek isolates. Two clusters were discern… Leer más
Velarde R., Porrero MC., Serrano E., Marco I., Garcia M., Tellez S., Dominguez L., Aymi R. y Lavin S.
Molecular diagnosis of lobomycosis-like disease in a bottlenose dolphin in captivity
Medical Mycology
50(1):106-9.
We report the diagnosis and molecular characterization of lobomycosis-like lesions in a captive bottlenose dolphin. The clinical picture and the absence of growth in conventional media resembled the features associated with Lacazia loboi. However sequencing of ribosomal DNA and further phylogenetic analyses showed a novel sequence more related to Paracoccidioides brasilensis than to L. loboi. Moreover, the morphology of the yeast cells differed from those L. loboi causing infections humans. These facts suggest that the dolphin lobomycosis-like lesions might have been be caused by different a different fungus clustered inside the order Onygenales. A successful treatment protocol based on topic and systemic terbinafine is also detailed.… Leer más
Esperon F., Garcia-Parraga D., Belliere EN. y Sanchez-Vizcaino JM..
Large-scale screening of a targeted Enterococcus faecalis mutant library identifies envelope fitness factors
PLoS ONE 6(12):e29023.
Spread of antibiotic resistance among bacteria responsible for nosocomial and community-acquired infections urges for novel therapeutic or prophylactic targets and for innovative pathogen-specific antibacterial compounds. Major challenges are posed by opportunistic pathogens belonging to the low GC% Gram-positive bacteria. Among those, Enterococcus faecalis is a leading cause of hospital-acquired infections associated with life-threatening issues and increased hospital costs. To better understand the molecular properties of enterococci that may be required for virulence, and that may explain the emergence of these bacteria in nosocomial infections, we performed the first large-scale functional analysis of E. faecalis V583, the first vancomy… Leer más
Rigottier-Gois L., Alberti A., Houel A., Taly JF., Palcy P., Manson J., Pinto D., Matos RC., Carrilero L., Montero N., Tariq M., Karsens H., Repp C., Kropec A., Budin-Verneuil A., Benachour A., Sauvageot N., Bizzini A., Gilmore MS., Bessieres P., Kok J., Huebner J., Lopes F., Gonzalez-Zorn B., Hartke A. y Serror P.
First case of erysipelas in a free-ranging bottlenose dolphin (Tursiops truncatus) stranded in the Mediterranean Sea
Diseases Of Aquatic Organisms 97: 167–170.
An adult female bottlenose dolphin (Tursiops truncatus) stranded alive and subsequently died several minutes later on the Mediterranean coast of Spain on 14 July 2010. Clinical examination revealed foam through the blowhole and rales upon lung auscultation. On venipuncture, the blood was abnormally dense and dark. Hematological and biochemical abnormalities included dehydration, leukocytosis (48600 leukocytes µl−1) characterized by neutrophilia (48200 neutrophils µl−1), and elevated bilirubin (4.38 mg dl−1), alanine aminotransferase (382.3 U l−1), aspartate aminotransferase (1449.3 U l−1), lactate dehydrogenase (1631.3 U l−1), and creatine kinase (404.7 U l−1)… Leer más
Melero-Asensio M., Rubio-Guerri C., Crespo JL., Arbelo M., Vela AI., Garcia-Parraga D., Sierra E., Dominguez L. y Sanchez-Vizcaino JM..
