Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates
1 de octubre de 2007
Artículo de investigación publicado en Journal of Clinical Microbiology
The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism
Castellanos E., Aranaz A., Romero B., de Juan L., Alvarez J., Bezos J., Rodriguez-Campos S., Stevenson K., Mateos A. y Dominguez L..
