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Últimas noticias de Investigación
1 de febrero de 2012
Vía publiDB
Chryseobacterium oncorhynchi sp. nov., isolated from rainbow trout (Oncorhynchus mykiss)
Investigación publicada en Systematic and Applied Microbiology
Genotypic and phenotypic analyses were performed on five Gram-negative, catalase and oxidasepositive, rod-shaped bacteria isolated from the gill and liver of four rainbow trout. Studies based on comparative 16S rRNA gene sequence analysis showed that the five new isolates shared 99.8–100% sequence similarity and that they belong to the genus Chryseobacterium. The nearest phylogenetic neighbours of the strain 701B-08T were Chryseobacterium ureilyticum F-Fue-04IIIaaaaT (99.1% 16S rRNA gene sequence similarity) and Chryseobacterium joosteii LMG 18212T (98.6%). DNA–DNA hybridization values between…
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1 de febrero de 2012
Vía publiDB
Tuberculosis due to Mycobacterium bovis and Mycobacterium caprae in sheep
Artículo de investigación publicado en The Veterinary Journal
Tuberculosis was diagnosed in three flocks of sheep in Galicia, Spain, in 2009 and 2010. Two flocks were infected with Mycobacterium bovis and one flock was infected with Mycobacterium caprae. Infection was confirmed by the comparative intradermal tuberculin test, bacteriology, molecular analysis and histopathology. Sheep have the potential to act as a reservoir for tuberculosis…
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1 de febrero de 2012
Vía publiDB
First isolation of the anamorph of Kazachstania heterogenica from a fatal infection in a primate host
Medical Mycology publica este artículo de investigación
We describe the isolation of the anamorph of the ascomycetous yeast Kazachstania heterogenica from a fatal infection in a 2 year, 9-month-old female white-handed gibbon (Hylobates lar). The yeast was observed in histological sections (lung and intestine) and co-isolated with the bacterium Escherichia coli from different internal organs. This is the first report of the recovery of this yeast from a fatal infection in a primate host…
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1 de febrero de 2012
Vía publiDB
Diagnosis of Tuberculosis in Camelids: Old Problems, Current Solutions and Future Challenges
Investigación publicada en Transboundary and Emerging Diseases
In spite of great efforts for its control and eradication, tuberculosis remains one of the most important zoonosis worldwide. Its causative agents, the members of the Mycobacterium tuberculosis complex, have a wide host range that complicates the epidemiology of this disease. Among susceptible species to these pathogens, camelids from the New World (llama, alpaca and vicuña) and Old World (Bactrian camel and dromedary) are acquiring an increasing importance in several European countries because of its growing number and could act as reservoirs of the disease for livestock and humans in their n…
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1 de febrero de 2012
Vía publiDB
Tuberculosis in goats: Assessment of current in vivo cell-mediated and antibody-based diagnostic assays
Artículo de investigación publicado en The Veterinary Journal
Tuberculosis in goats, mainly caused by Mycobacterium bovis and M. caprae, is a zoonotic disease with implications for public health, as well as having an economic impact due to decreased goat production, increased mortality rates and costs of diagnosis. There is an increasing need for surveillance of tuberculosis-infected goat herds, particularly in countries that are not officially free of bovine tuberculosis, and goats sharing farms with cattle should be subjected to the official tuberculin test. In Spain, some regions have programmes for the control of tuberculosis in goats, applying the s…
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27 de enero de 2012
Vía publiDB
Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats
Veterinary Microbiology publica este artículo de investigación
A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected …
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1 de enero de 2012
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Septicemic salmonellosis caused by Salmonella Hessarek in wintering and migrating song thrushes (Turdus philomelos) in Spain
Journal of Wildlife Diseases publica este artículo de investigación
We investigated two mortality events in wintering and migrating Song Thrushes (Turdus philomelos) in Catalonia, northeastern Spain in 2009 and 2010. Both episodes occurred in late February to mid-March during the spring migration. Salmonellosis produced by the serotype Salmonella enterica subsp. enterica serotype Hessarek (S. Hessarek) was identified as the cause of death in both episodes. Poor body condition, marked splenomegaly, and microscopic disseminated intravascular coagulation with numerous intravascular and tissular bacteria were the most consistent findings. Macro-restriction profili…
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1 de enero de 2012
Vía publiDB
Molecular diagnosis of lobomycosis-like disease in a bottlenose dolphin in captivity
Artículo de investigación publicado en Medical Mycology
We report the diagnosis and molecular characterization of lobomycosis-like lesions in a captive bottlenose dolphin. The clinical picture and the absence of growth in conventional media resembled the features associated with Lacazia loboi. However sequencing of ribosomal DNA and further phylogenetic analyses showed a novel sequence more related to Paracoccidioides brasilensis than to L. loboi. Moreover, the morphology of the yeast cells differed from those L. loboi causing infections humans. These facts suggest that the dolphin lobomycosis-like lesions might have been be caused by different a d…
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15 de diciembre de 2011
Sergio González Domínguez
El Laboratorio de referencia europeo para la tuberculosis bovina celebra su tercer workshop
El laboratorio de referencia europeo (EURL) para la tuberculosis bovina, ubicado en el Centro VISAVET de la Universidad Complutense de Madrid, organiza del 15 al 16 de diciembre de 2011 una reunión europea donde se estudiarán los avances obtenidos en las técnicas de diagnóstico y control de la enfermedad, así como los logros conseguidos en su estandarización
La erradicación de la infección en el ganado bovino por las especies del complejo Mycobacterium tuberculosis causantes de enfermedad sigue siendo uno de los objetivos pendientes de la Unión Europea, que anualmente dedica importantes esfuerzos en la reducción de los niveles de prevalencia e incidencia.
A pesar de los progresos alcanzados en lo referente al conocimiento de la patogenia de la enfermedad y a su posible diagnóstico, no ha podido alcanzarse su erradicación debido a una serie de factores que, en determinados contextos epidemiológicos, complican su control.
La variabilidad inherente a la prueba de la intradermotuberculinización, técnica de diagnóstico de primera línea en la lucha frente a la enfermedad, dificulta su estandarización, motivo por el cual son necesarios esfuerzos coordinados por parte de la comunidad científica y las administraciones para su optimización. De igual forma, el desarrollo de potenciales métodos de diagnóstico complementarios en determinadas situaciones abre nuevas posibilidades en el campo de la lucha frente a la enfermedad que deben ser evaluadas convenientemente.A la reunión acude representación de Dirección General de Salud y Consumidores de la Comisión Europea, del EURL, de 23 laboratorios nacionales de referencia (NRLs) de Europa así como del Ministerio de Medio Ambiente y Medio Rural y Marino de España.…
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15 de diciembre de 2011
Vía publiDB
Large-scale screening of a targeted Enterococcus faecalis mutant library identifies envelope fitness factors
PLoS ONE publica este artículo de investigación
Spread of antibiotic resistance among bacteria responsible for nosocomial and community-acquired infections urges for novel therapeutic or prophylactic targets and for innovative pathogen-specific antibacterial compounds. Major challenges are posed by opportunistic pathogens belonging to the low GC% Gram-positive bacteria. Among those, Enterococcus faecalis is a leading cause of hospital-acquired infections associated with life-threatening issues and increased hospital costs. To better understand the molecular properties of enterococci that may be required for virulence, and that may explain t…
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