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Últimas publicaciones científicas ISI
High spoligotype diversity within a Mycobacterium bovis population: Clues to understanding the demography of the pathogen in Europe
Veterinary Microbiology 141(1-2):89-95.
Mycobacterium bovis is the main causative agent of bovine tuberculosis. This zoonotic disease produces important economic losses and must be considered a threat to endangered animal species and public health. This study was performed (1) to assess the degree of diversity of the Spanish M. bovis isolates and its effect on the epidemiology of the infection, and (2) to understand the connection of M. bovis populations within a European context. In this report we resume the DVR-spoligotyping results of 6215 M. bovis isolates collected between 1992 and 2007 from different hosts. The isolates clustered into 252 spoligotypes which varied largely in frequency, geographical distribution and appearance in different animal species. In general, the mos… Leer más
Rodriguez S., Romero B., Bezos J., de Juan L., Alvarez J., Castellanos E., Moya N., Lozano F., Gonzalez S., Saez JL., Mateos A., Dominguez L. y Aranaz A.
Detection of bluetongue serotype 4 in mouflons (Ovis aries musimon) from Spain
Veterinary Microbiology 141:164-7.
Bluetongue serotype 4 (BTV4) has been detected for the first time in tissue samples from 2 mouflons (Ovis aries musimon) from the South of Spain, in a retrospective study. The samples included in this study had been fixed and paraffin-embedded for over a year prior to their analysis using a BTV group-specific and a BTV4-specific RT-PCR test. Lung and lymphatic nodes were found positive in both specimens. The amplified DNA was confirmed to be BTV4 by sequencing the RT-PCR products and comparing them with other sequences from GenBank. The combination of RNA extraction from paraffin-embedded samples and serotype-specific real-time RT-PCR assays provides the tools for the detection of BTV from samples stored for a long time. The results shown i… Leer más
Rodriguez B., Sanchez-Cordon PJ., Molina V., Risalde MA., Perez de Diego AC., Gomez-Villamandos JC. y Sanchez-Vizcaino JM.
Experimental infection with Mycobacterium caprae in goats and evaluation of immunological status in tuberculosis and paratuberculosis co-infected animals
Veterinary Immunology And Immunopathology 133 (2-4): 269-275.
Tuberculosis in goats (caused by Mycobacterium caprae and M. bovis) has become a significant concern in recent years because of its high prevalence in certain caprine herds in Spain and other European countries, and also due to the potential transmission to other animals and human beings. In the present study, a transthoracic model of tuberculosis infection was performed on goats. Animals were selected based on the serological response used to detect paratuberculosis in goats (negative and positive results). The kinetics of the immune response was evaluated using the interferon-gamma (IFN-gamma) assay, skin tests and serology of paratuberculosis during nine months post-challenge. At the end of the study the animals were necropsied, tubercul… Leer más
Bezos J., de Juan L., Romero B., Alvarez J., Mazzucchelli F., Mateos A., Dominguez L. y Aranaz A.
Resistance mechanisms and farm-level distribution of fecal Escherichia coli isolates resistant to extended-spectrum cephalosporins in pigs in Spain
Research In Veterinary Science 88:83-87.
INTRODUCTION: Fecal Escherichia coli isolates showing a phenotype of reduced susceptibility or resistance to extended-spectrum cephalosporins are common among pigs in Spain. The aim of this study was to describe the main beta-lactam resistance mechanisms carried by these strains and their distribution at farm-level. MATERIALS AND METHODS: Twenty-nine E. coli isolates showing reduced susceptibility or resistance to extended-spectrum cephalosporins were collected from a sampling frame of 80 pig farms distributed over 13 Spanish provinces. The survey was carried out at the slaughterhouse level in 2004. RESULTS: Of the 29 isolates, 21 (72%) met the criteria for a positive phenotypic confirmatory test for extended-spectrum beta-lactamases (ESBL)… Leer más
Escudero E., Vinue L., Teshager T., Torres C. y Moreno MA.
Avian mycobacteriosis in free-living raptors in Majorca Island, Spain
Avian Pathology : Journal Of The W.V.P.A 39(1), 1-6.
Avian mycobacteriosis is a chronic, infectious disease caused by different species of mycobacteria, usually belonging to the Mycobacterium avium complex. From 2004 to 2007, 589 raptors brought dead or sick to a wildlife rehabilitation centre in Majorca (Balearic Islands, Spain) were necropsied. The birds belonged to 12 different species, chiefly common kestrel (Falco tinnunculus) (n=297), scops owl (Otus scops) (n=109), barn owl (Tyto alba) (n=75), long-eared owl (Asio otus) (n=58), peregrine falcon (Falco peregrinus) (n=27), and booted eagle (Hieraaetus pennatus) (n=13). Gross lesions compatible with mycobacteriosis were observed in 14 birds (2.4%) found in several locations in Majorca. They were 12 kestrels (prevalence in this species, 4.… Leer más
Millan J., Negre N., Castellanos E., de Juan L., Mateos A., Parpal L. y Aranaz A.
Streptococcus porci sp. nov., isolated from swine sources
International Journal Of Systematic And Evolutionary Microbiology 60(Pt 1):104-8.
Two unidentified Gram-positive, catalase-negative, coccus-shaped organisms were recovered from pigs and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Streptococcus, although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequence studies confirmed this identification and showed that the nearest phylogenetic relatives of the unknown cocci were Streptococcus plurextorum 1956-02(T) and Streptococcus suis NCTC 10234(T) (97.9 and 96.0 % 16S rRNA gene sequence similarity, respectively). The new isolates were related most closely to S. suis CIP 103217(T) based on rpoB gene sequence analysis (&l… Leer más
Vela AI., Perez-Sancho M., Zamora L., Palacios L., Dominguez L. y Fernandez-Garayzabal JF.
Percentage of reactor animals to single comparative cervical intradermal tuberculin (SCCIT) in small ruminants in Punjab Pakistan
Acta Tropica 113(1):88-91.
To investigate the percentage reactor animals to SCCIT in sheep and goat the present study was carried out by using bovine and avian PPDs at 7 Livestock Experiment Stations of Punjab and villages around two cities. The overall percentage of reactor animals to SCCIT at farms in sheep and goat was 0.9% and 2.4%, while it was 0.4% and 0.0% around two cities, respectively. Hundred percent of goat and 86% of sheep farms under study had positive reactor animals. The reactor animals to SCCIT around two cities were 0% in goat and 0.4% in sheep. Goats (P<0.05) and sheep (P<0.001) kept with large ruminants at farms had higher percentage of reactor animals. Age showed significant (P<0.01) association with tuberculosis in sheep and goat. In go… Leer más
Javed MT., Munir A., Shahid M., Severi G., Irfan M., Aranaz A. y Cagiola M.
Unilateral scrotal pyocele in ram caused by Staphylococcus capitis
Australian Veterinary Journal 87(12):484-6.
A massive unilateral scrotal pyocele caused by Staphylococcus capitis in a 6-year-old ram is reported. Ultrasound examination of the right hemiscrotum showed an irregular hyperechoic mass in an anechoic fluid. A dense exudate was collected from the scrotum for microbiological analysis. Grossly, there was an extensive greenish purulent exudate and a completely atrophied right testis. Coagulase-negative S. capitis was isolated in pure culture. To the authors` knowledge, this is the first report of genital infection by S. capitis in rams. This microorganism should be included in the differential diagnosis of ovine genital infections… Leer más
Lacasta D., Ferrer LM., Ramos JJ., Ortin A., Vela AI. y Latre MV.
Novel genetic environment of qnrB2 associated with TEM-1 and SHV-12 on pB1004, an IncHI2 plasmid, in Salmonella Bredeney BB1047 from Spain
The Journal Of Antimicrobial Chemotherapy 64(6):1334-6.
Resistance to fluoroquinolones through plasmid-mediated qnr genes, especially when associated with resistance to cephalosporins by extended-spectrum b-lactamases (ESBLs), is an emerging phenomenon in enterobacteria. This is worrisome in the case of Salmonella, as these are two of the major antimicrobials used in invasive salmonellosis… Leer más
Gutierrez B., Herrera-Leon S., Escudero JA., Hidalgo L., Gonzalez-Sanz R., Arroyo M., San Millan A., Echeita MA. y Gonzalez-Zorn B.
First data on Eurasian wild boar response to oral immunization with BCG and challenge with a Mycobacterium bovis field strain
Vaccine 27(48):6662-8.
The Eurasian wild boar (Sus scrofa) is considered a reservoir for bovine tuberculosis (bTB) caused by Mycobacterium bovis and closely related members of the Mycobacterium tuberculosis complex in south-central Spain. The vaccination of wildlife with BCG offers an alternative to culling and to movement restriction for the control of bTB among wildlife reservoirs. In this study, we hypothesized that oral BCG immunization of wild boar would affect the expression of immunoregulatory genes and confer protection against M. bovis. Three groups were used to describe the infection, pathological findings and gene expression profiles in wild boar: BCG-vaccinated and M. bovis-challenged (vaccinated challenged group; N=6), non-vaccinated and M. bovis-cha… Leer más
Ballesteros C., Garrido JM., Vicente J., Romero B., Galindo RC., Minguijon E., Villar M., Martin-Hernando MP., Sevilla I., Juste R., Aranaz A., de la Fuente J. y Gortazar C.
