http://www.healthsurveillance.eu/ en-gb 2010 Health Surveillance Centre Health Surveillance Centre of the Complutense University Noticiashttp://www.vigilanciasanitaria.es/en/eurasian-wild-boar-response-to-skin-testing-with-mycobacterial-and-non-mycobacterial-antigens/37=384/Wed, 1 Sep 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/eurasian-wild-boar-response-to-skin-testing-with-mycobacterial-and-non-mycobacterial-antigens/37=384/Preventive veterinary medicine publish this investigation article

Eurasian wild boar (Sus scrofa) are able to maintain bovine tuberculosis (bTB) in the wild and are most probably able to transmit the disease to other species, thus acting as a true wildlife reservoir. Translocation of wild boar is a common practice in European countries. Therefore, identifying effective tools for bTB diagnosis in living wild boar is crucial for the implementation of control measu...]]>http://www.vigilanciasanitaria.es/en/polyclonal-aspergillus-fumigatus-infection-in-captive-penguins-/37=365/Thu, 26 Aug 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/polyclonal-aspergillus-fumigatus-infection-in-captive-penguins-/37=365/Investigation published in Veterinary microbiology

Aspergillus fumigatus is a known opportunistic pathogen of penguins. Most reports of penguin aspergillosis have focused on clinical, histopathological and microbiological findings. The molecular characterization of A. fumigatus isolates involved in invasive infections in penguins has yet to be addressed. The aim of this work was to study the possible coexistence of different A. fumigatus genotypes...]]>http://www.vigilanciasanitaria.es/en/a-simulation-model-for-the-potential-spread-of-foot-and-mouth-disease-in-the-castile-and-leon-region-of-spain/37=385/Sun, 1 Aug 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/a-simulation-model-for-the-potential-spread-of-foot-and-mouth-disease-in-the-castile-and-leon-region-of-spain/37=385/Investigation article published in Preventive veterinary medicine

A spatial stochastic model was used to simulate the spread of a foot-and-mouth disease (FMD) epidemic in the Castile-and-Leon (CyL) region of Spain. The model was fitted using information available on premises demographics and on assumptions for animal movements, indirect contacts, and airborne exposure. Control measures dictated by Spanish and European Union regulations constituted a reference st...]]>http://www.vigilanciasanitaria.es/en/presence-of-herpesvirus-in-striped-dolphins-stranded-during-the-cetacean-morbillivirus-epizootic-along-the-mediterranean-spanish-coast-in-2007/37=375/Sun, 1 Aug 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/presence-of-herpesvirus-in-striped-dolphins-stranded-during-the-cetacean-morbillivirus-epizootic-along-the-mediterranean-spanish-coast-in-2007/37=375/Archives of virology publish this investigation article

A screening for herpesvirus (HV) was carried out using a tissue bank obtained from the cetacean morbillivirus (CeMV) mortality episode that occurred along the Mediterranean Spanish coast in 2007. A total of 14 cetaceans, including six long-finned pilot whales and eight striped dolphins, were studied using histopathology and molecular analysis to detect HV and CeMV. In five of the eight dolphins (6...]]>http://www.vigilanciasanitaria.es/en/first-case-of-highly-pathogenic-avian-influenza-in-poultry-in-spain/37=387/Sun, 1 Aug 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/first-case-of-highly-pathogenic-avian-influenza-in-poultry-in-spain/37=387/Transboundary and emerging diseases publish this investigation article

Summary In October 2009, highly pathogenic avian influenza virus (HPAIV) was isolated for the first time in poultry in Spain. Sequencing analysis revealed that it was an H7N7 HPAIV. The progenitors of H7 HPAIV strains involved in recent European poultry outbreaks were simultaneously circulating in wild birds. The infected Spanish farm is located close to a reservoir abundant in wild birds. Epidemi...]]>http://www.vigilanciasanitaria.es/en/identifying-areas-for-infectious-animal-disease-surveillance-in-the-absence-of-population-data-highly-pathogenic-avian-influenza-in-wild-bird-populations-of-europe/37=391/Sun, 1 Aug 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/identifying-areas-for-infectious-animal-disease-surveillance-in-the-absence-of-population-data-highly-pathogenic-avian-influenza-in-wild-bird-populations-of-europe/37=391/Investigation article published in Preventive veterinary medicine

A large number (n=591) of H5N1 highly pathogenic avian influenza virus (HPAIV) outbreaks have been reported in wild birds of Europe from October 2005 through January 2009. Consequently, prevention and control strategies have been implemented in response to the outbreaks and considerable discussion has taken place regarding the need for implementing surveillance programs in high-risk areas with the...]]> http://www.vigilanciasanitaria.es/en/jornada-t-eacutecnica-sobre-prevenci-oacuten-y-control-de-listeria-monocytogenes/26=67/Fri, 30 Jul 2010 00:00:00 +0200Outreachhttp://www.vigilanciasanitaria.es/en/jornada-t-eacutecnica-sobre-prevenci-oacuten-y-control-de-listeria-monocytogenes/26=67/<p>La Oficina de Exportaci&oacute;n de la Carne de Espa&ntilde;a (OECE), en colaboraci&oacute;n con el Ministerio de Medio Ambiente y Medio Rural y Marino (MARM) y el Ministerio de Sanidad y Pol&iacute;tica Social, organizar&oacute;n esta jornada celebrada el 29 de julio</p>

<p>Lucas Dom&iacute;nguez moder&oacute; la mesa redonda donde se abordaron los aspectos t&eacute;cnicos m&aacute;s relevantes de inter&eacute;s para la industria, como la diversidad gen&eacute;tica y persistencia de <em>L. monocytogenes</em>, <em>Listeria</em> en biofilms, experiencias en limpieza, controles anal&iacute;ticos en f&aacute;brica y las posibilidades de las altas presiones</p>...]]>http://www.vigilanciasanitaria.es/en/experimental-infection-of-eurasian-wild-boar-with-mycobacterium-avium-subsp-avium-/37=361/Thu, 29 Jul 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/experimental-infection-of-eurasian-wild-boar-with-mycobacterium-avium-subsp-avium-/37=361/Investigation article published in Veterinary microbiology

The Eurasian wild boar (Sus scrofa) is increasingly relevant as a host for several pathogenic mycobacteria. We aimed to characterize the first experimental Mycobacterium avium subsp. avium (MAA) infection in wild boar in order to describe the lesions and the immune response as compared to uninfected controls. Twelve 1-4-month-old wild boar piglets were housed in class III bio-containment facilitie...]]>http://www.vigilanciasanitaria.es/en/molecular-characterization-of-mycobacterium-avium-subspecies-paratuberculosis-types-ii-and-iii-isolates-by-a-combination-of-miru-vntr-loci/37=358/Thu, 29 Jul 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/molecular-characterization-of-mycobacterium-avium-subspecies-paratuberculosis-types-ii-and-iii-isolates-by-a-combination-of-miru-vntr-loci/37=358/Investigation article published in Veterinary microbiology

Mycobacterial interspersed repetitive units and variable number tandem repeats typing (MIRU-VNTR) is a useful technique that has been recently applied to characterize members of the Mycobacterium avium complex (MAC). The aim of this study was to examine the genetic variability among a collection of Spanish M. avium subspecies paratuberculosis (M. a. paratuberculosis) isolates with a combination of...]]>http://www.vigilanciasanitaria.es/en/kinetic-analysis-of-ex-vivo-human-blood-infection-by-leishmania/37=373/Tue, 13 Jul 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/kinetic-analysis-of-ex-vivo-human-blood-infection-by-leishmania/37=373/Investigation article published in PLoS Neglected Tropical Diseases

The leishmanioses, vector-borne diseases caused by the trypanosomatid protozoan Leishmania, are transmitted to susceptible mammals by infected phlebotomine sand flies that inoculate promastigotes into hemorrhagic pools created in host skin. We assumed that promastigotes are delivered to a blood pool, and analyzed early promastigote interactions (0–5 min) with host components, which lead to parasit...]]>http://www.vigilanciasanitaria.es/en/comparison-of-sensitivity-potential-essays-of-different-european-tuberculin-ppd-in-cattle/72=54/Fri, 9 Jul 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/comparison-of-sensitivity-potential-essays-of-different-european-tuberculin-ppd-in-cattle/72=54/Presentation by Carmen Casal Comendador to obtain the Master of Research. Faculty of Veterinary Medicine, Complutense University of Madrid

La herramienta fundamental en la lucha contra la tuberculosis es el diagnóstico, ya que la detección de los animales positivos permite eliminarlos de la explotación, limitando en gran medida la fuente de infección para el resto animales. La prueba de diagnóstico oficial es la intradermotuberculinización (IDTB), consiste en la inoculación intradérmica de un derivado proteico purificado (PPD) obteni...]]>http://www.vigilanciasanitaria.es/en/molecular-characterization-of-mycobacterium-avium-subspecies-paratuberculosis-isolates-epidemiological-distribution-in-spain/72=53/Fri, 9 Jul 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/molecular-characterization-of-mycobacterium-avium-subspecies-paratuberculosis-isolates-epidemiological-distribution-in-spain/72=53/Elena Castellanos Rizaldos defended the PhD Thesis at the Faculty of Veterinary Medicine of de Complutense University of Madrid

Paratuberculosis or Johne’s disease is a chronic granulomatous inflammation that mainly affects the gastrointestinal tract of domestic ruminants, although it also affects other animal species. The etiological agent is M. a. paratuberculosis, a member of the Mycobacterium avium complex. The main objective of this thesis, which has been compiled in this summary, is the development and appl...]]>http://www.vigilanciasanitaria.es/en/mycobacterium-tuberculosis-infection-in-cattle/34=580/Sun, 4 Jul 2010 00:00:00 +0200Outreachhttp://www.vigilanciasanitaria.es/en/mycobacterium-tuberculosis-infection-in-cattle/34=580/Poster communication in 31st Annual Congress of the European Society of Mycobacteriology (ESM 2010)

According to the EU Bovine Tuberculosis Task Force, bovine tuberculosis is the infection in cattle caused by any mycobacterial species within the Mycobacterium tuberculosis complex. However, M. bovis is considered the main etiological agent of the disease in animals. Tuberculosis in cattle due to the human pathogen, M. tuberculosis, is a rare event but the detection of this infection is increasing...]]>http://www.vigilanciasanitaria.es/en/molecular-epidemiology-underlines-the-importance-of-mycobacterium-caprae-in-livestock-and-wildlife/34=582/Sun, 4 Jul 2010 00:00:00 +0200Outreachhttp://www.vigilanciasanitaria.es/en/molecular-epidemiology-underlines-the-importance-of-mycobacterium-caprae-in-livestock-and-wildlife/34=582/Oral communication in 31st Annual Congress of the European Society of Mycobacteriology (ESM 2010)

Mycobacterium caprae (M. caprae) is a member of the M. tuberculosis complex which was first described as the main etiological agent of caprine tuberculosis in Spain. However, this pathogen can also infect other animal species and human beings. In the present study we have characterised M. caprae isolates from 791 animals by DVR-spoligotyping. The diversity of spoligotypes based on the number of p...]]>http://www.vigilanciasanitaria.es/en/molecular-techniques-used-to-characterize-mycobacterium-avium-subspecies-paratuberculosis-an-up-date/34=578/Sun, 4 Jul 2010 00:00:00 +0200Outreachhttp://www.vigilanciasanitaria.es/en/molecular-techniques-used-to-characterize-mycobacterium-avium-subspecies-paratuberculosis-an-up-date/34=578/Poster communication in 31st Annual Congress of the European Society of Mycobacteriology (ESM 2010)

Mycobacterium avium subspecies paratuberculosis is a world-wide microorganism that causes paratuberculosis or Johne’s disease in many animal species, mainly ruminants. The role of this agent in the development of Crohn’s disease in humans has been discussed although this hypothesis still remains controversial. M. a. paratuberculosis is difficult to isolate due to long time incubation periods and ...]]>http://www.vigilanciasanitaria.es/en/gamma-inteferon-assay-for-tuberculosis-diagnosis-in-cattle-and-goats-study-of-factors-affecting-results-of-the-assay/34=579/Sun, 4 Jul 2010 00:00:00 +0200Outreachhttp://www.vigilanciasanitaria.es/en/gamma-inteferon-assay-for-tuberculosis-diagnosis-in-cattle-and-goats-study-of-factors-affecting-results-of-the-assay/34=579/Poster presented in 31st Annual Congress of the European Society of Mycobacteriology (ESM 2010)

The gamma-interferon assay is as a useful tool (together with the tuberculin test) for the diagnosis of tuberculosis in cattle and goats. In these experiments, we have evaluated the effect of factors such as phenol concentration and dialysis of PPDs, delay in processing samples and use of single or duplicate results on the performance of the assay. Moreover, we have evaluated the influence of tube...]]>http://www.vigilanciasanitaria.es/en/spanish-database-of-animal-mycobacteriosis-mycodb-application-in-epidemiological-studies/34=577/Sun, 4 Jul 2010 00:00:00 +0200Outreachhttp://www.vigilanciasanitaria.es/en/spanish-database-of-animal-mycobacteriosis-mycodb-application-in-epidemiological-studies/34=577/Conference in 31st Annual Congress of the European Society of Mycobacteriology (ESM 2010)

Epidemiological studies based on molecular characterization have allowed a better understanding of several factors as for example transmission between domestic and wildlife animals, animal movement, outbreaks, etc. The Direct Variable Repeat Spacer Oligonucleotide Typing technique or DVR-spoligotyping is based on polymorphism of the chromosomal DR locus, which consists of a variable number of dire...]]>http://www.vigilanciasanitaria.es/en/evaluating-surveillance-in-wild-birds-by-the-application-of-risk-assessment-of-avian-influenza-introduction-into-spain/37=381/Thu, 1 Jul 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/evaluating-surveillance-in-wild-birds-by-the-application-of-risk-assessment-of-avian-influenza-introduction-into-spain/37=381/Epidemiology and Infections publish this investigation article

Early detection of highly pathogenic avian influenza (HPAI) in its natural reservoirs is a prerequisite for preventing disease spread to humans. The risk of introduction of H5N1 HPAI was assessed in order to design a risk-based surveillance system in Spain. Areas at highest risk for H5N1 HPAI followed a northeast-southwest direction, with two significant clusters located in the north and the south...]]>http://www.vigilanciasanitaria.es/en/immunohistochemical-detection-of-bluetongue-virus-in-fixed-tissue/37=363/Thu, 1 Jul 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/immunohistochemical-detection-of-bluetongue-virus-in-fixed-tissue/37=363/Journal of Comparative Pathology publish this investigation article

The VP7 structural protein is the most abundant of the major core proteins and is highly conserved in all serotypes of bluetongue virus (BTV). The aim of this study was to develop immunohistochemical techniques for the detection of BTV VP7 in Bouin`s- and formalin-fixed and paraffin wax-embedded tissues from small ruminants (sheep and goats) naturally infected with BTV. Tissue samples were taken f...]]>http://www.vigilanciasanitaria.es/en/new-contributions-to-the-diagnosis-of-viral-diseases-in-apis-mellifera-and-their-application-to-the-epidemiological-study/72=55/Wed, 30 Jun 2010 00:00:00 +0200Investigationhttp://www.vigilanciasanitaria.es/en/new-contributions-to-the-diagnosis-of-viral-diseases-in-apis-mellifera-and-their-application-to-the-epidemiological-study/72=55/Deborah Kukielka Zunzunegui defended the PhD Thesis at the Faculty of Veterinary Medicine of de Complutense University of Madrid

Spain is the main honeybee producer country in Europe holding approximately 2.500.000 beehives. Honeybees are highly important insects due to the honeybee production and their use as natural pollinators of several agricultural products. Therefore, the problems they suffer affect beekeepers, the agricultural and the scientific community. Due to the increasing disappearance phenomena that Apis mel...]]>