Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types using real-time PCR and high resolution melt analysis of the MAP1506 gene
April 1st, 2010
Investigation published in Journal of Clinical Microbiology
High resolution melt (HRM) can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (Proline-Proline-Glutamic acid) gene family
Castellanos E., Aranaz A. and De Buck J.
