Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates
October 1st, 2007
Investigation article published in Journal of Clinical Microbiology
The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism
Castellanos E., Aranaz A., Romero B., de Juan L., Alvarez J., Bezos J., Rodriguez-Campos S., Stevenson K., Mateos A. and Dominguez L..
