PCR amplification and high resolution melt curve analysis as a rapid diagnostic method to genotype members of the Mycobacterium avium-intracellulare complex
In Press
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases publish this investigation article
Some of the members of the Mycobacterium avium-intracellulare (MAI) complex are recognised as human pathogens in both immunocompromised and immunocompetent patients. The current molecular methods that are available to genotype the MAI complex members can be both expensive and technically demanding. In this report, we describe for the first time the application of a real-time PCR and high resolution melt approach to differentiate between the complex members by targeting a member of the PPE (Pro-Pro-Glu) gene family, MACPPE24. In this aim, reference strains of the Mycobacterium avium subspecies and Mycobacterium intracellulare (M. intracellulare) were used to optimise the technique. Then, this real time PCR-HRM approach was used to distinguish 10 Mycobacterium avium subspecies hominissuis field isolates from the M. intracellulare reference strain
Castellanos E., Aranaz A. and De Buck J.
