News
September 1st, 2010
Via publiDB
Eurasian wild boar response to skin-testing with mycobacterial and non-mycobacterial antigens
Preventive veterinary medicine publish this investigation article
Eurasian wild boar (Sus scrofa) are able to maintain bovine tuberculosis (bTB) in the wild and are most probably able to transmit the disease to other species, thus acting as a true wildlife reservoir. Translocation of wild boar is a common practice in European countries. Therefore, identifying effective tools for bTB diagnosis in living wild boar is crucial for the implementation of control measures. We describe for the first time the sex and origin related differences in the skin-test response to mycobacterial antigens (bPPD and aPPD) and to a non-mycobacterial antigen (PHA, a plant derived …
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August 26th, 2010
Via publiDB
Polyclonal Aspergillus fumigatus infection in captive penguins
Investigation published in Veterinary microbiology
Aspergillus fumigatus is a known opportunistic pathogen of penguins. Most reports of penguin aspergillosis have focused on clinical, histopathological and microbiological findings. The molecular characterization of A. fumigatus isolates involved in invasive infections in penguins has yet to be addressed. The aim of this work was to study the possible coexistence of different A. fumigatus genotypes in five clinical cases of invasive aspergillosis in captive penguins. Differences in other relevant characteristics of the isolates, including mating type and invasiveness, were also considered.
A…
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August 1st, 2010
Via publiDB
A simulation model for the potential spread of foot-and-mouth disease in the Castile and Leon region of Spain
Investigation article published in Preventive veterinary medicine
A spatial stochastic model was used to simulate the spread of a foot-and-mouth disease (FMD) epidemic in the Castile-and-Leon (CyL) region of Spain. The model was fitted using information available on premises demographics and on assumptions for animal movements, indirect contacts, and airborne exposure. Control measures dictated by Spanish and European Union regulations constituted a reference strategy to which six alternative control strategies were compared. For the reference strategy, the median (95% PI) numbers of infected, depopulated, and quarantined premises were 141 (2-1099), 164 (4-1…
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August 1st, 2010
Via publiDB
Presence of herpesvirus in striped dolphins stranded during the cetacean morbillivirus epizootic along the Mediterranean Spanish coast in 2007
Archives of virology publish this investigation article
A screening for herpesvirus (HV) was carried out using a tissue bank obtained from the cetacean morbillivirus (CeMV) mortality episode that occurred along the Mediterranean Spanish coast in 2007. A total of 14 cetaceans, including six long-finned pilot whales and eight striped dolphins, were studied using histopathology and molecular analysis to detect HV and CeMV. In five of the eight dolphins (62.5%) infected with CeMV, eight novel HV sequences were also detected. No HV lesions were found in any of the coinfected dolphins, which may indicate that HV did not contribute to the mortality in the…
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August 1st, 2010
Via publiDB
First Case of Highly Pathogenic Avian Influenza in Poultry in Spain
Transboundary and emerging diseases publish this investigation article
Summary In October 2009, highly pathogenic avian influenza virus (HPAIV) was isolated for the first time in poultry in Spain. Sequencing analysis revealed that it was an H7N7 HPAIV. The progenitors of H7 HPAIV strains involved in recent European poultry outbreaks were simultaneously circulating in wild birds. The infected Spanish farm is located close to a reservoir abundant in wild birds. Epidemiological investigation found no links to other poultry holdings and those located in the control area were negative for AIV. Previous spatial risk analyses had identified the area where the infected h…
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August 1st, 2010
Via publiDB
Identifying areas for infectious animal disease surveillance in the absence of population data: highly pathogenic avian influenza in wild bird populations of Europe
Investigation article published in Preventive veterinary medicine
A large number (n=591) of H5N1 highly pathogenic avian influenza virus (HPAIV) outbreaks have been reported in wild birds of Europe from October 2005 through January 2009. Consequently, prevention and control strategies have been implemented in response to the outbreaks and considerable discussion has taken place regarding the need for implementing surveillance programs in high-risk areas with the objective of early detecting and preventing HPAIV epidemics. However countries ability to define the temporal and spatial extension of the high risk areas has been impaired by the lack of information…
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July 30th, 2010
Sergio González
Jornada técnica sobre prevención y control de Listeria monocytogenes
La Oficina de Exportación de la Carne de España (OECE), en colaboración con el Ministerio de Medio Ambiente y Medio Rural y Marino (MARM) y el Ministerio de Sanidad y Política Social, organizarón esta jornada celebrada el 29 de julio
Lucas Domínguez moderó la mesa redonda donde se abordaron los aspectos técnicos más relevantes de interés para la industria, como la diversidad genética y persistencia de L. monocytogenes, Listeria en biofilms, experiencias en limpieza, controles analíticos en fábrica y las posibilidades de las altas presiones…
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July 29th, 2010
Via publiDB
Experimental infection of Eurasian wild boar with Mycobacterium avium subsp. avium
Investigation article published in Veterinary microbiology
The Eurasian wild boar (Sus scrofa) is increasingly relevant as a host for several pathogenic mycobacteria. We aimed to characterize the first experimental Mycobacterium avium subsp. avium (MAA) infection in wild boar in order to describe the lesions and the immune response as compared to uninfected controls. Twelve 1-4-month-old wild boar piglets were housed in class III bio-containment facilities. Four concentrations of MAA suspension were used: 10, 10(2) and 10(4) mycobacteria (2 animals each, oropharyngeal route) and 2.5x10(6) mycobacteria (2 animals each by the oropharyngeal and nasal rou…
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July 29th, 2010
Via publiDB
Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci
Investigation article published in Veterinary microbiology
Mycobacterial interspersed repetitive units and variable number tandem repeats typing (MIRU-VNTR) is a useful technique that has been recently applied to characterize members of the Mycobacterium avium complex (MAC). The aim of this study was to examine the genetic variability among a collection of Spanish M. avium subspecies paratuberculosis (M. a. paratuberculosis) isolates with a combination of MIRU-VNTR loci. For this purpose we tested six MIRU-VNTR loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32, VNTR-292 and VNTR-259) in 70 M. a. paratuberculosis isolates of Types II and III that were recovered f…
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July 13rd, 2010
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Kinetic analysis of ex vivo human blood infection by Leishmania
Investigation article published in PLoS Neglected Tropical Diseases
The leishmanioses, vector-borne diseases caused by the trypanosomatid protozoan Leishmania, are transmitted to susceptible mammals by infected phlebotomine sand flies that inoculate promastigotes into hemorrhagic pools created in host skin. We assumed that promastigotes are delivered to a blood pool, and analyzed early promastigote interactions (0–5 min) with host components, which lead to parasite endocytosis by blood leukocytes, and to host infection. Promastigotes were incubated with NHS or with heparinized blood in near-physiological conditions, and we used cell radioimmunoassay and flow c…
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