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July, 2010 news


July 30th, 2010

Sergio González


Technical Conference about Prevention and Control of the Listeria monocytogenes

Technical Conference about Prevention and Control of the Listeria monocytogenes

The Spanish Meet Export Office (OECE), in collaboration with the Ministry of Environment and Rural und Marine Affairs (MARM), and the Ministry of Social Affairs and Health,  organized this Conference, which took place on 29th July el 29



Lucas Domínguez moderated the roundtable where it spoke about the most important technical aspects, centered in the interest to the industry, such as genetic diversity and persistence of L. monocytogenes, Listeria in biofilms, cleaning experience, analytical controls at the factory and the possibilities of high pressure Read more





July 29th, 2010

Via publiDB


Veterinary Microbiology

Experimental infection of Eurasian wild boar with Mycobacterium avium subsp. avium

Investigation article published in Veterinary Microbiology


The Eurasian wild boar (Sus scrofa) is increasingly relevant as a host for several pathogenic mycobacteria. We aimed to characterize the first experimental Mycobacterium avium subsp. avium (MAA) infection in wild boar in order to describe the lesions and the immune response as compared to uninfected controls. Twelve 1-4-month-old wild boar piglets were housed in class III bio-containment facilities. Four concentrations of MAA suspension were used: 10, 10(2) and 10(4) mycobacteria (2 animals each, oropharyngeal route) and 2.5x10(6) mycobacteria (2 animals each by the oropharyngeal and nasal rou… Read more




July 29th, 2010

Via publiDB


Veterinary Microbiology

Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci

Investigation article published in Veterinary Microbiology


Mycobacterial interspersed repetitive units and variable number tandem repeats typing (MIRU-VNTR) is a useful technique that has been recently applied to characterize members of the Mycobacterium avium complex (MAC). The aim of this study was to examine the genetic variability among a collection of Spanish M. avium subspecies paratuberculosis (M. a. paratuberculosis) isolates with a combination of MIRU-VNTR loci. For this purpose we tested six MIRU-VNTR loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32, VNTR-292 and VNTR-259) in 70 M. a. paratuberculosis isolates of Types II and III that were recovered f… Read more




July 21st, 2010

Via publiDB


21st International Pig Veterinary Society Congress

VISAVET participated in this event with the next communications:


- African swine fever: a high risk again

- African swine fever threats: are we ready?



July 13rd, 2010

Via publiDB


PLoS Neglected Tropical Diseases

Kinetic analysis of ex vivo human blood infection by Leishmania

Investigation article published in PLoS Neglected Tropical Diseases


The leishmanioses, vector-borne diseases caused by the trypanosomatid protozoan Leishmania, are transmitted to susceptible mammals by infected phlebotomine sand flies that inoculate promastigotes into hemorrhagic pools created in host skin. We assumed that promastigotes are delivered to a blood pool, and analyzed early promastigote interactions (0–5 min) with host components, which lead to parasite endocytosis by blood leukocytes, and to host infection. Promastigotes were incubated with NHS or with heparinized blood in near-physiological conditions, and we used cell radioimmunoassay and flow c… Read more




July 9th, 2010

Via publiDB


Carmen Casal Comendador

Comparison of sensitivity (potential essays) of different European tuberculin PPD in cattle

Presentation by Carmen Casal Comendador to obtain the Master of Research. Faculty of Veterinary Medicine, Complutense University of Madrid


La herramienta fundamental en la lucha contra la tuberculosis es el diagnóstico, ya que la detección de los animales positivos permite eliminarlos de la explotación, limitando en gran medida la fuente de infección para el resto animales. La prueba de diagnóstico oficial es la intradermotuberculinización (IDTB), consiste en la inoculación intradérmica de un derivado proteico purificado (PPD) obtenido a partir de M. bovis (en la prueba realizada en humanos, la PPD se obtiene a partir de M. tuberculosis). Es imprescindible que la PPD empleada en la práctica cumpla una serie de características res… Read more




July 9th, 2010

Via publiDB


Elena Castellanos Rizaldos

Molecular characterization of Mycobacterium avium subspecies paratuberculosis isolates. Epidemiological distribution in Spain

Elena Castellanos Rizaldos defended the PhD Thesis at the Faculty of Veterinary Medicine of the Complutense University of Madrid


Paratuberculosis or Johne’s disease is a chronic granulomatous inflammation that mainly affects the gastrointestinal tract of domestic ruminants, although it also affects other animal species. The etiological agent is M. a. paratuberculosis, a member of the Mycobacterium avium complex. The main objective of this thesis, which has been compiled in this summary, is the development and application of new rapid molecular tools to characterize M. a. paratuberculosis. This research is mainly focused on the description of new methodologies capable of discriminating the most slow-growing phe… Read more




July 1st, 2010

Via publiDB


Epidemiology and Infection

Evaluating surveillance in wild birds by the application of risk assessment of avian influenza introduction into Spain

Epidemiology and Infection publish this investigation article


Early detection of highly pathogenic avian influenza (HPAI) in its natural reservoirs is a prerequisite for preventing disease spread to humans. The risk of introduction of H5N1 HPAI was assessed in order to design a risk-based surveillance system in Spain. Areas at highest risk for H5N1 HPAI followed a northeast-southwest direction, with two significant clusters located in the north and the southwest of the country. Most (83%) of the veterinary units (VUs) obtained fewer samples than would have been expected if samples had been collected using a risk-based design. In October 2009, a HPAI outb… Read more




July 1st, 2010

Via publiDB


Journal of Comparative Pathology

Immunohistochemical Detection of Bluetongue Virus in Fixed Tissue

Journal of Comparative Pathology publish this investigation article


The VP7 structural protein is the most abundant of the major core proteins and is highly conserved in all serotypes of bluetongue virus (BTV). The aim of this study was to develop immunohistochemical techniques for the detection of BTV VP7 in Bouin`s- and formalin-fixed and paraffin wax-embedded tissues from small ruminants (sheep and goats) naturally infected with BTV. Tissue samples were taken from animals in which BTV infection had been confirmed by reverse transcriptase polymerase chain reaction. Optimal results were obtained by incubation of monoclonal antibody 2E9 on samples fixed with B… Read more







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