March, 2008 news
March 15th, 2008
Via publiDB
PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae
Investigation article published in Microbiological research
Streptococcus phocae, a bacterial pathogen of seals, could reliably be identified by PCR amplification using oligonucleotide primers designed according to species specific segments of the previously sequenced 16S rRNA gene and the 16S-23S rDNA intergenic spacer region of this species. The PCR mediated assay allowed an identification of S. phocae isolated from harbor and gray seals and from Atlantic salmons. No cross-reaction could be observed with 13 different other streptococcal species and subspecies and with Lactococcus garvieae strains investigated for control purposes…
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March 7th, 2008
Via publiDB
Información de la cadena alimentaria en productos de origen animal
Conference in Curso de porcino
March 4th, 2008
Sergio González Domínguez
V Edition of the Master in Food Security
The wide and changing food safety regulations,
requires continuous training of professionals
In October 2008 starts the fifth edition of the Master in Food Security of the College of Veterinarians of Madrid. The preregistration period is from 3 March to 19 September…
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March 1st, 2008
Via publiDB
Application of fluorescence in situ hybridization technique to detect simazine degrading bacteria in soil samples
Investigation published in Chemosphere
We propose a new approach to evaluate the natural attenuation capacity of soil by using fluorescence in situ hybridization (FISH). A specific oligonucleotide probe AtzB1 was designed based on the sequence data of the atzB gene involved in the hydrolytic deamination of s-triazines; this gene, located in a multiple copy plasmid was detected by the optimized FISH protocol. Two agricultural soils (Lodi and Henares) with a history of simazine treatments, and two natural soils (Soto and Monza), without previous exposure to simazine, were studied. AtzB1 probe-target cells were found only in the agric…
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March 1st, 2008
Via publiDB
Role of hepatic macrophages during the viral haemorrhagic fever induced by African Swine Fever Virus
Investigation article published in Histology and histopathology
To ascertain the role played by the various liver monocyte-macrophage populations in the course of a viral hemorrhagic fever, fifteen pigs were inoculated intramuscularly with the highly virulent isolate of African Swine Fever Virus (ASFV) España-70 and slaughtered at 1-7 days post-inoculation (dpi). Samples of liver were fixed in different solutions and routinely processed for morphological, immunohistochemical and ultrastructural studies. Viral antigen (vp73) was detected from 3 dpi onward, mainly in circulating monocytes of sinusoid and Kupffer\'s cells (KC), as well as in portal macrophage…
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March 1st, 2008
Via publiDB
Vaccines against bluetongue in Europe
Investigation published in Comparative immunology, microbiology and infectious diseases
After the incursion of bluetongue virus (BTV) into European Mediterranean countries in 1998, vaccination was used in an effort to minimize direct economic losses to animal production, reduce virus circulation and allow safe movements of animals from endemic areas. Vaccination strategies in different countries were developed according to their individual policies, the geographic distribution of the incurring serotypes of BTV and the availability of appropriate vaccines. Four monovalent modified live virus (MLV) vaccines were imported from South Africa and subsequently used extensively in both c…
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