Home \ Research \ Scientific publications \


Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations


October 1st, 2012

Investigation article published in Research in Veterinary Science


The aim of this study was to evaluate the specificity of the most widely used tuberculosis (TB) diagnostic tests, single intradermal tuberculin (SIT) and single comparative intradermal tuberculin (SCIT) tests and interferon-gamma (IFN-&#947;) assay in 937 animals from eight TB-free caprine flocks under different epidemiological situations. Maximum specificity was found using SCIT test (99.4-100% depending on the interpretation criteria) while SIT test and IFN-&#947; assay showed a slightly lower overall specificity (97.6-99.2% and 96.4-98.4%respectively). Specificity of the SIT test in a C.pseudotuberculosis infected flock was significantly (P < 0.05) lower if a severe interpretation criterion was applied. Similarly, specificity values of SIT test and particularly IFN-&#947; assay in a paratuberculosis (PTB)-vaccinated flock were lower than those observed in non-vaccinated flocks. Higher proportion of false positive reactors to TB tests (SIT and IFN-&#947; assay) were observed among animals positive in the PTB-ELISA in PTB vaccinated flock. These results demonstrate that TB diagnostic tests show an adequate specificity when performed in goats from TB-free flocks in most situations. However, certain factors such as C. pseudotuberculosis infection and paratuberculosis vaccination can have a negative impact in the most sensitive tests




Bezos J., Alvarez J., Minguez O., Marques S., Martin O., Vigo V., Pieltain C., Romero B., Rodriguez-Campos S., Casal C., Mateos A., Dominguez L. and de Juan L..




See this article
Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations

See it on NLM PubMed
Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations





Research in Veterinary Science
FACTOR YEAR Q
1.774 2012

NLMID: 401300

PMID: 22079129

ISSN: 0034-5288



TITLE: Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations


JOURNAL: Res. Vet. Sci.


NUMERACIÓN: 93(2):636-40


AÑO: 2012


PUBLISHER: Elsevier


AUTHORS: Bezos J., Alvarez J., Minguez O., Marques S., Martin O., Vigo V., Pieltain C., Romero B., Rodriguez-Campos S., Casal C., Mateos A., Dominguez L. and de Juan L..


DOI: http://dx.doi.org/ 10.1016/j.rvsc.2011.10.009


CITE THIS PUBLICATION:

Bezos J., Alvarez J., Minguez O., Marques S., Martin O., Vigo V., Pieltain C., Romero B., Rodriguez-Campos S., Casal C., Mateos A., Dominguez L. and de Juan L.. Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations. Research in Veterinary Science. 93(2):636-40. 2012. ISSN: 0034-5288. DOI: 10.1016/j.rvsc.2011.10.009