April 27^th, 2009

Perez de Diego AC., Sanchez-Matamoros A. and Sanchez-Vizcaino JM.

Conference in VIII Congreso de Ciencias Veterinarias y Biomédicas: Facultad de Veterinaria

Cytokines are protein molecules that cells from the immune system secrete in response to immune stimulation. There are different techniques to study their production and their action on individuals. This study consists of the development of a real time RT-PCR method for the detection of the expression of mRNA coding for six cytokines (TNF-α, IFNγ, IL-4, IL2, IL-10, IL-12). The preliminary results were obtained from the study of lymphocites stimulated with Con A and LPS, showing the detection of ARNm, IL-4, IL-2, IFNγ and TNF-α, IL-12, IL-10, respectively. Its subsequent application in sheep that were vaccinated against Chlamydophila

Cytokines gene expression detection by real time RT-PCR in sheep

Publication: Cytokines gene expression detection by real time RT-PCR in sheep

Link to VIII Congreso de Ciencias Veterinarias y Biomédicas: Facultad de Veterinaria